Simultaneous Determination of Eleven Quinolones Antibacterial Residues in Marine Products and Animal Tissues by Liquid Chromatography with Fluorescence Detection
CHUI-SHIANG CHANG1,2, WEI-HSIEN WANG1,3 AND CHIN-EN TSAI4*
1. Asia-Pacific Ocean Research Center, Department of Marine Biotechnology and Resources,
National Sun Yat-Sen University, Kaohsiung, Taiwan (R.O.C.)
2. Kaohsiung Branch, Bureau of Standards, Metrology and Inspection, MOEA, Kaohsiung, Taiwan (R.O.C.)
3. National Museum of Marine Biology and Aquarium, Pingtung, Taiwan (R.O.C.)
4. Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan (R.O.C.)
(Received: May 6, 2008; Accepted: September 18, 2008)
ABSTRACT
A simple and efficient multiresidue method was developed for determining 11 quinolones (QNs; marbofloxacin, norfloxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, nalidixic acid and flumequine) in chicken, pork, fish and shrimp. The analytes were extracted with 0.3% metaphosphoric acid and acetonitrile (1:1, v/v), followed by a HLB cartridge clean-up procedure. The HPLC separation was carried out on a symmetry column C-18 (250 mm × 4.5 mm i.d., 5 μm) with linear gradient elution of 0.1% formic acid and acetonitrile as mobile phase and programmable fluorescence detection. The method was validated by spiking blank animals tissues at three different levels (25, 50 and 250 ng/g; except 6.25, 12.5 and 62.5 ng/g for DAN) while the linearity, detection limit, quantification limit, precision and accuracy were checked. Mean recoveries of 11 QNs from edible animal tissues were 71.7-105.3%. The limits of quantification in different muscle tissues ranged from 5.0 to 28.0 ng/g. The results showed this method was simple, rapid, sensitive and suitable for routine tests.
Key words: Quinolones, residue, HPLC