Analysis of Bioactive Triterpenes in Eriobotrya japonica LINDL. by High-Performance Liquid Chromatography
HUI-YA HO1, WEN-CHUAN LIN2, SUSUMU KITANAKA3, CHWEN-TZUEI CHANG4 AND JIN-BIN WU1*
1. Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung, Taiwan (R.O.C.)
2. Department of Pharmacology, China Medical University, Taichung, Taiwan (R.O.C.)
3. College of Pharmacy, Nihon University, Chiba 274-8555, Japan
4. Department of Internal Medicine Division of Endocrinology and Metabolism,
China Medical University Hospital, Taichung, Taiwan (R.O.C.)
(Received: March 26, 2008; Accepted: September 9, 2008)
ABSTRACT
Eriobotrya japonica (Rosaceae) has been used for clearing away heat in the lung to stop cough and lowering the adverse flow of gi to stop vomiting. High-performance liquid chromatographic method was applied to assay the five triterpenes contents in E. japonica from various locations. Samples were analyzed on a HyPURITY C-18 column (5 μm, 4.6 × 250 mm) eluted at a rate of 0.5 mL/min with a solvent of A - B (A, Methanol; B, 0.15% acetic acid aqueous; A: B = 85: 15, v/v). Triterpenes were detected by refractive index detection (RID). Regression equations of the calibration curves revealed good linear relationships (correlation coefficients: 0.9998-1.0000). The recoveries ranged between 98.39% - 102.41% and indicated good accuracy.
Key words: Eriobotrya japonica, HPLC, tormentic acid, maslinic acid, corosolic acid, oleanolic acid, ursolic acid