Determination of Aldicarb, Propoxur, Carbofuran, Carbaryl and Methiocarb Residues in Honey by HPLC with Post-column Derivatization and Fluorescence Detection after Elution from a Florisil Column
Feride Koc1*, Yusuf Yigit2, Yavuz Kursad Das3, Yasemin Gurel2 and Cevdet Yarali4
1.Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Atatürk University, Erzurum 25400, Turkey
2.Central Veterinary Control and Research Institute, Toxicology Laboratory, Ankara, Turkey
3.Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, 19 Mayis University, Samsun, Turkey
4.Central Veterinary Control and Research Institute Genetic Laboratory, Ankara, Turkey
(Received: march 20, 2007; Accepted: September 16, 2007)
ABSTRACT
The aim of this study was to develop an easy analytical method for determining aldicarb, propoxur, carbofuran, carbaryl and methiocarb residues in honey by high performance liquid chromatography with post-column derivatization and fluorescence detection (HPLC/PCD-FL) for routine analysis. A florisil packed column was used in sample preparation step and showed good performance. The recoveries ranged between 72.02 and 92.02% for various spiking levels (50, 100, 200 ng/g). The limits of detection were 4–5 ng/g, depending on different N-methylcarbamate pesticides. The extraction method used in the present study was easy, inexpensive, and rapid. Thus, it could be used efficiently in the routine monitoring studies.
Key words: honey, liquid chromatography, post-column derivatization, N-methylcarbamate pesticide, residue, florisil