M. R. Hadjmohammadi* and V. Sharifi

Department of Chemistry, University of Mazandaran, Babolsar, Iran

(Received: August 24, 2006; Accepted: March 22, 2007)

Abstract

   A solid phase extraction method for sample clean-up and preconcentration of vitamin B₁₂ in multivitamin tablet and its determination by HPLC-UV, UV and atomic absorption spectrophotometry is reported.  Solid phase parameters such as maximum loading capacity and breakthrough volume were 400 µg and 1750 mL of 0.001 ppm for this vitamin respectively.  A proper mobile phase used for HPLC was water/acetonitrile (75:25, v/v) with pH = 3.5 (adjusted with acetate buffer) at 25°C. Limit of detection and linear dynamic range for HPLC, UV and atomic absorption were 0.1, 0.1-25.0; 0.4, 0.4 -15.0; 2.7, 2.7-300.0 (μg/mL) respectively.  Recovery of vitamin B₁₂ was about 96%.

   A solid phase extraction method for sample clean-up and preconcentration of vitamin B in multivitamin tablet and its determination by HPLC-UV, UV and atomic absorption spectrophotometry is reported.  Solid phase parameters such as maximum loading capacity and breakthrough volume were 400 µg and 1750 mL of 0.001 ppm for this vitamin respectively.  A proper mobile phase used for HPLC was water/acetonitrile (75:25, v/v) with pH = 3.5 (adjusted with acetate buffer) at 25°C. Limit of detection and linear dynamic range for HPLC, UV and atomic absorption were 0.1, 0.1-25.0; 0.4, 0.4 -15.0; 2.7, 2.7-300.0 (μg/mL) respectively.  Recovery of vitamin B was about 96%.