Method development and validation for the high-performance liquid chromatography assay of gastrodin in water extracts from different sources of Gastrodia elata Blume

 

Wei-Cheng Chen ^a, Yi-Syuan Lai ^a, Kuan-Hung Lu ^a, Shih-Hang Lin ^a,
Li-Yun Liao ^b, Chi-Tang Ho ^c, Lee-Yan Sheen ^a,b,d,*

^a Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan
^b Center for Food and Biomolecules, National Taiwan University, Taipei, Taiwan
^c Department of Food Science, Rutgers University, New Brunswick, NJ, USA
^d National Center for Food Safety Education and Research, National Taiwan University, Taipei, Taiwan

 

Gastrodia elata Blume is commonly used as a medical herb in China for ameliorating headaches, dizziness, and convulsions. In previous studies, water extracts of G. elata Bl. (WGE) have demonstrated potential to act as therapeutic agents to improve depression-like symptoms in rats. As gastrodin (GAS) is a major active compound in WGE, its quantitation in WGE is important for quality control. The objective of this study was to develop an optimized and validated reversed-phase high-performance liquid chromatography method for the analysis of GAS in different sources of WGE. We evaluated the GAS content in varieties of G. elata Bl. including G. elata Bl. f. glauca S. Chow and G. elata Bl. f. elata. We also evaluated the GAS content of the latter variety from two different origins, Yun-nan and Hu-nan. The results indicate that the amount of GAS analyzed in WGE from G. elata Bl. f. glauca S. Chow is five times higher than that of G. elata Bl. f. elata from Yun-nan and Hu-nan. A significant difference in GAS content was observed between varieties of G. elata Bl., although not between locations of origin.

 

Keywords: Gastrodia elata Blume, gastrodin, method validation, quality control