Preparation of total flavonoids from loquat flower and its protective effect on acute alcohol-induced liver injury in mice

Shao-Kang Wu, Na Zhang, Xian-Rong Shen, Wei-Wei Mei, Ying He, Wei-Hong Ge

This study aimed to research the preparation techniques of total flavones from loquat flower (TFLF), its anti-oxidation capacity, and its protective effect on hepatic injury. The best extraction parameters by orthogonal experimentation were water at 100°C, extraction time 2.5 hours, solid/liquid ratio 1:20, and three decoctions. The chromogenic reaction to the flavones showed that loquat flowers mainly contained flavone, flavonol, and flavanone compounds combining ortho-phenolic hydroxyl group structure in the 10–30% ethanol fraction. The anti-oxidant capacity of O 2 − · was 26.09% and of OH−·was 83.01% by salicylic acid and pyrogallol auto-oxidation. Compared with the model group, TFLF lowered the levels of alanine aminotransferase, aspartate aminotransferase, triglyceride, and malondialdehyde and liver index significantly, and upregulated the expression of adipose triglyceride lipase and Heine oxygenase-1 mRNA. The present findings suggest that TFLF has protective effect on acute alcoholinduced liver injury in mice and may be related to its antioxidant and free-radical scavenging activity.

Keywords: antioxidant; extraction; hepatoprotection; identification; loquat flower; total flavones

Journal of Food and Drug Analysis, Volume 23, Issue 1, March 2015, Pages 136-143, ISSN 1021-9498, http://dx.doi.org/10.1016/j.jfda.2014.07.001. (http://www.sciencedirect.com/science/article/pii/S1021949814000775)