Development of two reference materials for all trans-retinol, retinyl palmitate, α- and γ-tocopherol in milk powder and infant formula

Adel B. Shehata, Mahmoud S. Rizk, Ahmad M. Farag, Ibrahim F. Tahoun

Vitamins are important food constituents that can be present in almost every foodstuff. Food quality and safety depends on food surveillance by reliable quantitative analysis enabled by appropriate quality control. Certified matrix reference materials are versatile tools to support quality assurance and control. However, in the case of vitamins, which are important in various foods, there is a lack of matrix reference materials. Two certified reference materials for the determination of all–trans-retinol, retinyl palmitate, and α- and γ-tocopherol in milk powder and infant formula have been developed by the National Institute of Standards, Egypt. This article presents the preparation, characterization, homogeneity, and stability testing as well as statistical treatment of data and certified value assignment. The assignment of the certified values and associated uncertainties in the prepared natural-matrix reference materials were based on the widely used approach of combining data from independent and reliable analytical methods.

Keywords: certification; infant formula; milk powder; reference materials; statistical analysis; vitamins

Journal of Food and Drug Analysis, Volume 23, Issue 1, March 2015, Pages 82-92, ISSN 1021-9498, http://dx.doi.org/10.1016/j.jfda.2014.10.004.,(http://www.sciencedirect.com/science/article/pii/S1021949814001367)