Dietary Resveratrol Modulates Metabolic Functions in Skeletal Muscle Cells
JACQUES KAMINSKI1, ALLAN LANÇON1, ESMERINA TILI2, VIRGINIE AIRES1, JEAN DEMARQUOY1, GERARD LIZARD1, JEAN-JACQUES MICHAILLE I 1,2 AND NORBERT LATRUFFE1＊
1. Laboratory of Biochemistry of Metabolism and Nutrition, University of Burgundy, Dijon, France
2. Ohio State University, Department of Molecular Virology, Immunology and Medical Genetics, Biological Research Tower , Columbus,
U.S.A.
ABSTRACT
Resveratrol is one of the best known polyphenol. While its effect on endothelial blood vessel cells, cancer cells, inflammatory processes and neurodegenerative events is well documented, only little is established on the metabolic implication of this phytophenol, particularly on skeletal muscle cells. Here, we report the effect of resveratrol on mouse skeletal muscle cells (C2C12) by measurement of cell proliferation, expression of metabolic genes and their transcription factor dependency, and modulation of non-coding microRNA expression. Resveratrol slightly decreases cell proliferation, while it up regulates PGC-1 a PPAR transcription factor co-activator involved in the control of metabolic genes. It also modulates microRNAs which are expressed in skeletal muscle cells and are involved in differentiation or contraction-relaxation. Results put forward new regulatory properties of resveratrol on skeletal muscles and highlight new potential applications of this molecule in muscle performances.
Key words: Resveratrol, metabolic functions, C2C12 skeletal muscle cells, microRNA