Chemical Fingerprint and Quantitative Analysis of Cistanche Deserticola by HPLC-DAD-ESI-MS
ZHEN-YA YANG1, DAN-YI LU1, SHUN YAO2, RONG-RONG ZHANG1, ZHENG-JIN JIANG1
AND ZHI-GUO MA1,3*
1. College of Pharmacy, Jinan University, Guangzhou, 510632, China
2. Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, 610065, China
3. Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Guangzhou, 510632, China
(Received: May 15, 2012; Accepted: September 24, 2012)
ABSTRACT
An HPLC-DAD-ESI-MS method was employed for the chromatographic fingerprint analysis of Cistanche deserticola (Roucongrong in Chinese). Eleven batches of samples were analyzed to establish the reference fingerprint of C. deserticola. Eight peaks in the fingerprint of all the 11 batches of samples were assigned as “characteristic peaks”, and identified by comparing their retention time and mass spectra with those of the reference substances. The similarity of the 11 batches of samples was evaluated by a simulative mean chromatogram. The results indicated that the samples from different origins shared similar HPLC fingerprints. In addition, an HPLC-DAD method was developed for simultaneous determination of the contents of the eight compounds in C. deserticola. All eight compounds showed good linear regression (R2 > 0.9998) within test ranges and the recovery of the method was in the range of 93.65 - 109.79%, indicating that the developed method combining chromatographic fingerprint with quantification analysis could readily be applied for the quality control of C. deserticola.
Key words: chromatographic fingerprint, quantitative analysis, Cistanche deserticola, HPLC-DAD-ESI-MS