Determination of Rutin and Puerarin in Teas and Pharmaceutical Preparations Using Poly (Evans Blue) Film-Modified Electrodes

JUNXIA WANG, JINGNAN MU, JING MA, YONG YANG, MAOQING WANG, LEI ZHU AND XIAOYAN DU^*

PublicHealthCollege, HarbinMedicalUniversity, Harbin, P. R. China

(Received: November 9, 2011; Accepted: March 12, 2012)

ABSTRACT

Single-walled carbon nanotubes (SWCNTs)/poly (Evans blue)(poly-EB) film-modified glassy carbon (GC) electrodes were fabricated for the voltammetric determination of rutin and puerarin in teas and pharmaceutical preparations. The SWCNTs/poly-EB film-modified electrode showed excellent electrocatalytic activity towards the oxidation of rutin and puerarin. Two well-defined oxidation peaks of rutin and puerarin appeared at 510 and 810 mV, respectively. The peak current depended linearly on the concentration of rutin and puerarin in the range from 1.6 × 10-7 to 2.0 × 10-5 mol/L and 3.0 × 10-7 to 4.6 × 10-5 mol/L, respectively, with the detection limit of 8.2 × 10-8 mol/L for rutin and 1.2 × 10-7 mol/L for puerarin. The modified electrode was successfully applied to the determination of rutin and puerarin in teas and pharmaceutical preparations.

Key words: poly (Evans blue), carbon nanotubes, modified electrode, rutin, puerarin