Gastroprotective Effects of Ping-Wei San on Indomethacin-Induced Gastric Ulcer
CHENG TIONG1, WEN-LI LIANG2, HSIANG-YIN LIU2, KUN-TENG WANG2,3,
CHUN-CHAO CHANG1 AND CHING-CHIUNG WANG2*
1. Division of Gastroenterology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C.
2. School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan, R.O.C.
3. Division of Research and Analysis, Food and Drug Administration, Department of Health, Executive Yuan, Taiwan, R.O.C.
(Received: January 11, 2011; Accepted: August 19, 2011)
ABSTRACT
Ping-Wei San (PWS), a Chinese medical decoction, was used to improve digestive absorption. This study was aimed to determine whether treatment with PWS reduce gastric ulceration induced by indomethacin and influencing its anti-inflammatory effect. PWS was extracted with boiling water and the decoction was freeze-dried into powder (H-PWS). The amounts of glycyrrhizin and hesperidin in H-PWS were analyzed by a HPLC method to be 38 and 19 mg/g, respectively. Co-administration of H-PWS resulted in a dose-dependent inhibition of indomethacin-induced gastric mucosal cell death. H-PWS (200 mg/kg) also alleviated the indomethacin-induced gastric ulcer in male Wistar rats in vivo. The inhibition effects of NO and PGE2 production were enhanced by indomethancin co-treatment with H-PWS from LPS-stimulated RAW 264.7 cells. H-PWS (200 mg/kg) also augmented the anti-inflammatory effect of indomethacin in 1% carrageen-induced paw edema. Moreover, H-PWS co-treatment with indomethacin produced a lower PGE2 level in serum than only indomethacin. Based on the results, co-treatment with H-PWS reduced the occurrence of indomethacin-induced gastric ulcers without affecting the anti-inflammatory effect of indomethacin. Therefore, we suggested that H-PWS can be developed as an anti-ulcer drug and H-PWS could be taken with indomethacin to decrease ulceration.
Key words: Ping-Wei San, Indomethacin, Gastric ulcer, Anti-inflammation