Using Orthogonal Arrays to Obtain Efficient and Reproducible Extraction Conditions of Geniposide and Genipin in Gardenia Fruit with Liquid Chromatography-Mass Spectrometry Determinations
SHAU-CHUN WANG1*, SHU-CHI LEE1, CHIA-HUNG HUNG1, HUI-JU LIAO1,
CHIH-MIN HUANG1 AND TUNG-HU TSAI2
1. Department of Chemistry and Biochemistry, National Chung Cheng University, Chia-Yi, Taiwan, R.O.C.
2. Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
(Received: October 22, 2010; Accepted: June 24, 2011)
ABSTRACT
The conditions to efficiently and reproducibly extract geniposide and genipin in Gardenia fruit powders were established using Taguchi experimental design approaches to improve extraction recovery. Geniposide, the major iridoid glycoside component of Gardenia fruits, which has been recognized to have choleretic effects, is transformed to Genipin in animals. In this study, the extraction conditions of geniposide and genipin such as solvent types, solvent acidities and boiling times were investigated under the guidance of experimental designs to obtain the highest content in extracts determined with liquid chromatography-mass spectrometry (LC-MS) methods. Herbal powders ground from Gardenia fruits, Gardenia jasminoides Ellis and Gardenia jasminoides Ellis var. grandiflora Nakai, were used. This paper has successfully demonstrated the use of Taguchi orthogonal arrays to obtain proper extraction conditions of geniposide and genipin in the above fruits. These design methods are capable of obtaining the optimum extraction conditions in only nine trials in which three factors, namely solvent type, boiling time and solvent acidity are investigated. The results show that solvent acidity is the influencing factor in achieving adequate contents and the other factors only have minor influences. The highest content obtained is similar to the reported data. In addition, using Taguchi orthogonal arrays within four experimental trials has identified that the extraction of ground fine powders results in more reproducible recovery than that of coarse ones.
Key words: orthogonal array, Geniposide, Genipin, LC-MS, Gardenia fruit