Preparation of a Naltrexone HCl Potentiometric Sensor and Its Application to Pharmaceutical Analysis and Drug Determination in Biological Fluids
FATEMEH GHORBANI-BIDKORBEH1, SAEED SHAHROKHIAN2,3, ALI MOHAMMADI4*
AND RASSOUL DINARVAND5
1. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Chemistry, Sharif University of Technology, Tehran, Iran
3. Institute for Nanoscience and Technology, Sharif University of Technology, Tehran, Iran
4. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
5. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
(Received: May 10, 2010; Accepted: June 22, 2011)
ABSTRACT
A novel ion selective electrode is fabricated for naltrexone HCl and used in pharmaceutical analysis and drug determination in biological fluids without complicated pretreatments and extractions, using direct potentiometry. The naltrexone complex with sodium tetraphenyl borate (NaTPB) is obtained by in situ soaking PVC membrane electrode in an 1 × 10-3 M naltrexone solution. The sensor exhibited fast, reproducible and linear sub-Nernstian response over concentration range of 1 × 10-5 - 1 × 10-3 M with a detection limit of 5 × 10-6 M. The membrane sensor was successfully applied to the determination of naltrexone in capsules as well as for its determination in urine and plasma samples.
Key words: Naltrexone HCl, ion selective electrode, direct potentiometry, pharmaceutical analysis, biological fluids