Study on The Contents of Aristololactams in Piper wallichii and Their Renal Cytotoxicities
ANHUA WEI, DAONIAN ZHOU, JINLAN RUAN*, YALING CAI, CHAOMEI XIONG,
GUANGHUA WU AND HUIBIN LIU
Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province,
College of Pharmacy, Huazhong University of Science and Technology, Wuhan, China
(Received: October 26, 2010; Accepted: May 28, 2011)
ABSTRACT
In the study, three aristololactams were isolated from Piper wallichii and determined simultaneously by a HPLC method using a C18 column with a gradient elution of methanol and water. The content ranges of the target compounds in twelve batches of Piper wallichii were 0.100 - 0.110 mg/g dry weight (cepharanone B), 0.198 - 0.208 mg/g dry weight (aristolactam AII), and 0.047 - 0.059 mg/g dry weight (aristololactam AIIIa), respectively. Their anti-proliferative effects in HK-2 cells were determined by MTT assay and cells membrane damage were assessed by LDH release assay. Results indicated that the test compounds showed a time- and dose-dependent cytotoxicity in HK-2 cells, and the values of IC50 were cepharanone B > aristolactam AII > aristololactam AIIIa.
Key words: Piper wallichii, Aristololactams, HPLC, Cytotoxicities