Preventive Effect of Methanol Extract from Agrocybe cylindracea (MEAC) Mushroom on the Cooking Oil
Fumes-Induced Expressions of Cyclooxygenase-2 via Suppression of Nuclear Factor-kappaB (NF–kB) in CL-3 Cells
LIEN-HUI WANG1, HUEI LEE2, TAI-AN CHIANG3, MEI-JU CHEN4 AND SHWU-YUANN LIN5*
1. Department of Food Nutrition, Chung Hwa University of Medical Technology,
89 Wen-Hwa 1st St., Jen-Te Shiang, Tainan Hsien, Taiwan. R.O.C.
2. Institute of Medical and Molecular Toxicology, Chung Shan Medical University,
110 Section 1st. Chien-Kuo N. Rd., Taichung City, Taiwan. R.O.C.
3. Graduate Institute of Biological Science and Technology, Department of Medical Technology, Chung Hwa University of
Medical Technology, 89 Wen-Hwa 1st St., Jen-Te Shiang, Tainan Hsien, Taiwan. R.O.C.
4. Department of Long-Term Care, Chung Hwa University of Medical Technology,
89 Wen-Hwa 1st St., Jen-Te Shiang, Tainan Hsien, Taiwan. R.O.C.
5. Department of Hospitality Management, Chung Hwa University of Medical Technology,
89 Wen-Hwa 1st St., Jen-Te Shiang, Tainan Hsien, Taiwan. R.O.C.
(Received: June 13, 2010; Accepted: April 29, 2011)
ABSTRACT
Methanol extract from Agrocybe cylindracea strain B (MEAC) mushroom is reported to suppress reactive oxygen species (ROS) production and DNA oxidative damage induced by cooking oil fumes (COF). This study examined the effects of MEAC on COF-stimulated expression of the cyclooxygenase-2 (COX-2) and cytochrome P450 CYP1A1 genes in human lung adenocarcinoma CL-3 cells. MEAC did not alter COF-stimulated CYP1A1 mRNA expression. However, MEAC significantly inhibited COF (100 μg/mL) stimulation of COX-2 mRNA and protein levels in a dose-dependent manner. The concentration of MEAC that decreased COF-stimulated COX-2 protein expression by 50% was 500 μg/mL. These data suggest that MEAC selectively affects particular types of the COF-mediated genes. COX-2 is reported to be regulated by the nuclear factor-kappaB (NF-kB) pathway. The gel retardation assay was used and showed that MEAC alone did not alter the basal NF-kB DNA binding activity; in the presence of COF, it dose-dependently suppressed COF-increased activity of NF-kB. These data suggest that MEAC may exert its anti-COF action on COX-2 gene expression via the NF-kB pathway. The results of this study support that MEAC mediates the COF-stimulated ROS production and DNA damage in CL-3 lung cancer cells.
Key words: cooking oil fumes, Agrocybe cylindracea, COX-2, nuclear factor-kappaB