Evaluation of Antioxidants Using an Arachidonic Acid-Stimulated Platelet Electron Spin Resonance System
KUO-HSIEN HSIEH1,2#, JIE-JEN LEE2,3#, LIN-WEN LEE4#, Joen-RONG sHEU2,4,
Su-TZY HWANG4 and Duen-Suey Chou2*
1.Department of Orthopedics, Tung’s Taichung MetroHarbor Hospital, Taichung, Taiwan, R.O.C.
2.Department of Pharmacology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C.
3.Department of Surgery, Mackay Memorial Hospital and Mackay Medical college, Taipei, Taiwan, R.O.C.
4.Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan, R.O.C.
(Received: September 17, 2010; Accepted: March 21, 2011)
ABSTRACT
A novel cell-containing assay system using electron spin resonance (ESR) and spin-trapping methods was established. We used this assay system to evaluate the antioxidant activities of various dietary antioxidants including resveratrol, lycopene, quercetin and rutin. Resveratrol showed the strongest radical-scavenging activity among the compounds tested. Our results provided useful data for the establishment of a model for evaluating the antioxidant activities of natural dietary substances in cell-containing systems.
Key words: arachidonic acid, platelets, electron spin resonance, hydroxyl radical, carbon-centered radical