Resveratrol Treatment Attenuates the Wound-Induced Inflammation in Zebrafish Larvae through the
Suppression of Myeloperoxidase Expression
YUN-FENG LIAO1, MIN-CHUAN CHIOU2, JEN-NING TSAI3, CHI-CHUNG WEN4,
YUN-HSIN WANG5, CHIEN-CHUNG CHENG2* AND YAU-HUNG CHEN1*
1. Department of Chemistry, Tamkang University, Tamsui, Taiwan, R.O.C.
2. Department of Applied Chemistry, National Chia-Yi University, Chia-Yi, Taiwan, R.O.C.
3. School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, R.O.C.
4. Department of Mathematics, Tamkang University, Tamsui, Taiwan, R.O.C.
5. Division of Basic Research, Koo Foundation Sun Yat-Sen Cancer Center, Taipei, Taiwan, R.O.C.
(Received: September 3, 2010; Accepted: March 9, 2011)
ABSTRACT
Resveratrol, a polyphenolic phytoalexin found in many plants, was reported to exhibit anti-inflammatory effects, but its molecular mechanism is not fully understood. This study was aimed to investigate the anti-inflammatory effects of resveratrol in vivo using a zebrafish model of wound-induced inflammation. Caudal fin-wounded zebrafish larvae were treated with resveratrol for 8 h. Neutrophil recruitment was visualized in transgenic line “Tg (mpx：GFP)” expressing GFP-tagged neutrophil-specific myeloperoxidase (mpx). The enzymatic activity of Mpx was evaluated by histochemical staining. Relative mRNA levels of mpx and cyclooxegenase-2 (cox2) were quantified by qRT-PCR, and the protein expression levels of Mpx and Cox2 were detected by immunostaining. Results showed that wound-induced neutrophil recruitment in zebrafish was not affected by resveratrol, but Mpx enzymatic activity in zebrafish was significantly suppressed by resveratrol in a dose-dependent manner. Moreover, both mRNA and protein expression levels of Mpx and Cox2 were significantly down-regulated by resveratrol. Taken together, our results provide in vivo evidence that the anti-inflammatory effects of resveratrol on wound-induced inflammation are significantly mediated through the suppression of Mpx and Cox2 at both transcriptional and protein levels, rather than the down-regulation of neutrophil recruitment. In conclusion, this in vivo zebrafish model can be readily applied to screen other potential anti-inflammatory compounds at a whole-organism level.
Key words: cyclooxygenase, inflammation, myeloperoxidase, resveratrol, zebrafish