Molybdate Assisted Ninhydrin Based Sensitive Analytical System for the Estimation of Drugs Containing Amine Group
PADMARAJAIAH NAGARAJA*, ASHWINEE KUMAR SHRESTHA,
ANANTHARAMAN SHIVAKUMAR AND NAEF GHALLAB SAEED Al-TAYAR
Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore 570 006, India
(Received: January 25, 2010; Accepted: January 11, 2011)
ABSTRACT
A sensitive spectrophotometric method for the analysis of isoniazid, lisinopril dihydrate, amoxicillin trihydrate, ampicillin trihydrate, glucosamine sulfate, phenylpropanolamine hydrochloride and gabapentin is described. The analysis is based on the reaction of drug molecules with ninhydrin and sodium molybdate mixture to give Ruhemann’s purple product with maximum absorbance (λmax) at 570 nm. The statistical analysis of intra-day and inter-day estimation of drugs as well as comparison with reported methods demonstrated high precision and accuracy of the proposed method. The method was successfully applied to the analysis of pharmaceutical preparations. The procedure was suitable for quality control application.
Key words: Ninhydrin, sodium molybdate, pharmaceutical analysis, quality control, spectrophotometry