Effect of Shengmai San on Insulin Resistance, Tumor Necrosis Factor-Alpha and Oxidative Stress in Rats Fed on a High-Fat Diet
HSIEN-TSUNG YAO1*, MENG-TSAN CHIANG2, CHONG-KUEI LII1, YI-WEI CHANG3,
SU-HUEI HSIEH4, JIA-HSUAN LIN1, RUEY-HWANG CHOU5,6 AND TENG-KUANG YEH4*
1. Department of Nutrition, China Medical University, 91, Hsueh-Shih Road, Taichung, 404, Taiwan, R.O.C.
2. Department of Food Science, National Taiwan Ocean University, 2, Pei-Ning Road, Keelung, 202, Taiwan, R.O.C.
3. SunTen Phytotech Co., Taipei 235, Taiwan, R.O.C.
4. Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes,
35, Keyan Road, Zhunan Town, Miaoli 350, Taiwan, R.O.C.
5. Center for Molecular Medicine, China Medical University Hospital, 2, Yuh-Der Road, Taichung 404, Taiwan, R.O.C.
6. Department of Biotechnology, Asia University, 505, Lioufeng Road, Taichung, 413, Taiwan, R.O.C.
(Received: August 17, 2010; Accepted: November 29, 2010)
ABSTRACT
Shengmai San (SMS) is a well-known traditional Chinese medicine formula prescribed for patients with coronary heart disease (CHD). Insulin resistance is the most likely explanation for the development and progression of this disease. To investigate the effect of SMS on high-fat diet-induced insulin resistance, inflammatory cytokine production and oxidative stress, male Wistar rats were fed with low-fat control diet, high-fat diet and high-fat diet supplemented with 4% SMS for eight weeks. An oral glucose tolerance test was conducted in the seventh week. Rats fed the SMS diet had significantly lower plasma fructosamine concentration (p < 0.05) and tended to have lower (p < 0.1) AUC values of plasma glucose and insulin concentrations after glucose challenge, compared to those fed the high-fat diet. Moreover, SMS reduced TNF-α and lipid peroxidation levels in the liver and heart. However, SMS had no effect on fasting plasma concentrations of glucose, insulin, triglyceride, non-esterified fatty acids, total cholesterol, HDL-cholesterol and insulin resistance index (homeostasis model assessment; HOMA) in rats. Our results show that SMS may have little or no significant effect on reducing insulin resistance, but display anti-oxidative and anti-inflammatory properties.
Key words: shengmai san, insulin resistance, high-fat diet, TNF-α, rats