Molecular Authentication of the Chinese Herb Huajuhong and Related Medicinal Material by DNA Sequencing and ISSR Markers
CHANG SU1, KA-LOK WONG2, PAUL PUI-HAY BUT2,4, WEI-WEI SU1 AND PANG-CHUI SHAW1,2,3*
1. School of Life Science, Sun Yat-sen University, Guangzhou, China
2. Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China
3. Department of Biochemistry, The Chinese University of Hong Kong, Hong Kong, China
4. Department of Biology, The Chinese University of Hong Kong, Hong Kong, China
(Received: November 16, 2009; Accepted: March 29, 2010)
ABSTRACT
DNA sequences in the chloroplast (trnH-psbA intergenic spacer) and nuclear (ITS) regions were amplified and determined for Huajuhong derived from the peels of the immature fruits of Citrus grandis ‘Tomentosa’ and related medicinal material. These sequences together with the inter-simple sequence repeats (ISSR) markers may be used to differentiate C. grandis ‘Tomentosa’ from other Citrus variants for the prevention of misuse.
Key words: pomelo peel, Citrus, Rutaceae, authentication, trnH-psbA, intergenic spacer (ITS), inter-simple sequence repeats (ISSR)