Detection of BPDE-DNA adducts in human umbilical cord blood by LC-MS/MS analysis
Ling Guo a,b,c,1, Xiao Jiang a,b, Hao-Yuan Tian a,b, Shang-Jin Yao a,b,c, Bo-Ya Li a,b, Rong-Jie Zhang d, Shu-Sheng Zhang c, Xin Sun a,b,*
a Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Chinese Center
for Disease Control and Prevention, 100050 Beijing, China
b National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, 100050 Beijing, China
c Department of Chemistry, Zhengzhou University, Zhengzhou 450001, China
d Henan Center for Disease Control and Prevention, Zhengzhou 450016, China
Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and 42 birth defect cases. We determined that there is no significant difference in the level of BPDE-dG formation between the normal and birth defect groups. This represents the first time to use an LC-MS/MS method to quantify BPDE-dG in human umbilical blood samples. The results indicated that under experimental conditions, BPDE-dG adducts were detected in all the human umbilical cord blood samples from the normal and birth defect groups.
Keywords: BPDE-dG, Human biomonitoring, LC-MS/MS, Molecular epidemiology